A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present...http://www.google.com.tw/patents/US6372497?utm_source=gb-gplus-share專利 US6372497 - Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
